Describe Any Experimental Controls Used in the Investigation
To screen for clones with the desired edit we used PCR amplification and Sanger sequencing of 264 bp around the targeted region. The manipulative experiment is considered an indispensable method for the investigation of the Rs response to a warming world since it effectively predicts the causal effects of climate change on soils Yuan et al 2017.
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To avoid any unintended effects of the silent mutation on protein expression we consulted the human codon usage frequency table to select a codon of similar usage frequency Dhindsa et al 2020.
. Many such experiments have been conducted over recent decades to examine the responses of grassland Rs to climate warming.
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